4H9X

Structure of Geobacillus kaustophilus lactonase, mutant E101G/R230C/D266N with Zn2+ and bound N-butyryl-DL-homoserine lactone

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Phosphotriesterase	polymer	330	36752.8	2	UniProt (Q5KZU5) Pfam (PF02126) In PDB	Geobacillus kaustophilus
2	A, B	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
3	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	A, B	HYDROXIDE ION	non-polymer		17.0	2	Chemie (OH)
5	A, B	N-[(3S)-2-oxotetrahydrofuran-3-yl]butanamide	non-polymer		171.2	2	Chemie (HL4)
6		water	water		18.0	189	Chemie (HOH)

Sequence modifications

A, B: 1 - 326 (UniProt: Q5KZU5)

PDB	External Database	Details
Gly -3	-	EXPRESSION TAG
Ser -2	-	EXPRESSION TAG
His -1	-	EXPRESSION TAG
Asn 0	-	EXPRESSION TAG
Gly 101	Glu 101	ENGINEERED MUTATION
Cys 230	Arg 230	ENGINEERED MUTATION
Asn 266	Asp 266	ENGINEERED MUTATION

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	73505.7
Non-Polymers*	Number of molecules	8
	Total formula weight	618.9
All*	Total formula weight	74124.6

*Water molecules are not included.