4H9T
Structure of Geobacillus kaustophilus lactonase, mutant E101N with bound N-butyryl-DL-homoserine lactone
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Phosphotriesterase | polymer | 330 | 36863.9 | 2 | UniProt (Q5KZU5) Pfam (PF02126) | Geobacillus kaustophilus | |
| 2 | C, F (A, B) | FE (III) ION | non-polymer | 55.8 | 2 | Chemie (FE) | |||
| 3 | D, G (A, B) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
| 4 | E, H (A, B) | N-[(3S)-2-oxotetrahydrofuran-3-yl]butanamide | non-polymer | 171.2 | 2 | Chemie (HL4) | |||
| 5 | I, J (A, B) | water | water | 18.0 | 341 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 326 (UniProt: Q5KZU5)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| His -1 | - | expression tag |
| Asn 0 | - | expression tag |
| Asn 101 | Glu 101 | engineered mutation |
| Asn 266 | Asp 266 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 73727.9 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 564.0 | |
| All* | Total formula weight | 74291.8 |
