4H7K

Crystal structure of haloalkane dehalogenase LinB I253M mutant from Sphingobium sp. MI1205

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Haloalkane dehalogenase	polymer	302	34114.6	1	UniProt (A4PEU6) Pfam (PF00561) In PDB	Sphingobium
2	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
3	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4		water	water		18.0	268	Chemie (HOH)

Sequence modifications

A: 1 - 296 (UniProt: A4PEU6)

PDB	External Database	Details
Met 253	Ile 253	engineered mutation
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag
His 302	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	34114.6
Non-Polymers*	Number of molecules	3
	Total formula weight	111.0
All*	Total formula weight	34225.6

*Water molecules are not included.