4H6S

Crystal structure of thrombin mutant E14eA/D14lA/E18A/S195A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Prothrombin	polymer	31	3545.0	1	UniProt (P00734) Pfam (PF09396) In PDB	Homo sapiens (human)	Coagulation factor II, Activation peptide fragment 1, Activation peptide fragment 2, Thrombin light chain, Thrombin heavy chain
2	B	Prothrombin	polymer	259	29706.2	1	UniProt (P00734) Pfam (PF00089) In PDB	Homo sapiens (human)	Coagulation factor II, Activation peptide fragment 1, Activation peptide fragment 2, Thrombin light chain, Thrombin heavy chain
3	B	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
4		water	water		18.0	103	Chemie (HOH)

Sequence modifications

A: 1 - 15 (UniProt: P00734)

PDB	External Database	Details
Ala 14	Glu 354	engineered mutation
Ala 14	Asp 361	engineered mutation

B: 16 - 247 (UniProt: P00734)

PDB	External Database	Details
Ala 18	Glu 366	engineered mutation
Ala 195	Ser 568	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	33251.2
Non-Polymers*	Number of molecules	1
	Total formula weight	23.0
All*	Total formula weight	33274.2

*Water molecules are not included.