4H62

Structure of the Saccharomyces cerevisiae Mediator subcomplex Med17C/Med11C/Med22C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	K	Mediator of RNA polymerase II transcription subunit 11	polymer	40	4341.6	1	UniProt (Q99278) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Mediator complex subunit 11
2	V	Mediator of RNA polymerase II transcription subunit 22	polymer	31	3404.7	1	UniProt (P32570) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Mediator complex subunit 22, Suppressor of RNA polymerase B 6
3	Q	Mediator of RNA polymerase II transcription subunit 17	polymer	312	36552.3	1	UniProt (P32569) Pfam (PF10156) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Mediator complex subunit 17, Suppressor of RNA polymerase B 4
4	Q	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)

Sequence modifications

K: 84 - 115 (UniProt: Q99278)

PDB	External Database	Details
Gly 80	-	EXPRESSION TAG
Ser 81	-	EXPRESSION TAG
His 82	-	EXPRESSION TAG
Mse 83	-	EXPRESSION TAG
Gly 116	-	LINKER
Ala 117	-	LINKER
Gly 118	-	LINKER
Ser 119	-	LINKER

V: 96 - 121 (UniProt: P32570)

PDB	External Database	Details
Gly 91	-	LINKER
Ala 92	-	LINKER
Gly 93	-	LINKER
Ser 94	-	LINKER
Gly 95	-	LINKER

Q: 377 - 687 (UniProt: P32569)

PDB	External Database	Details
Mse 376	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	44298.7
Non-Polymers*	Number of molecules	1
	Total formula weight	195.2
All*	Total formula weight	44493.9

*Water molecules are not included.