4H27

Modulating the function of human serine racemase and human serine dehydratase by protein engineering

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	L-serine dehydratase/L-threonine deaminase	polymer	364	38707.6	1	UniProt (P20132) Pfam (PF00291)	Homo sapiens (human)	SDH, L-serine deaminase, L-threonine dehydratase, TDH
2	B, C, D (A)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
3	E (A)	water	water		18.0	462	Chemie (HOH)

Sequence modifications

A: 1 - 328 (UniProt: P20132)

PDB	External Database	Details
Met -35	-	EXPRESSION TAG
Gly -34	-	EXPRESSION TAG
Ser -33	-	EXPRESSION TAG
Ser -32	-	EXPRESSION TAG
His -31	-	EXPRESSION TAG
His -30	-	EXPRESSION TAG
His -29	-	EXPRESSION TAG
His -28	-	EXPRESSION TAG
His -27	-	EXPRESSION TAG
His -26	-	EXPRESSION TAG
Ser -25	-	EXPRESSION TAG
Ser -24	-	EXPRESSION TAG
Gly -23	-	EXPRESSION TAG
Leu -22	-	EXPRESSION TAG
Val -21	-	EXPRESSION TAG
Pro -20	-	EXPRESSION TAG
Arg -19	-	EXPRESSION TAG
Gly -18	-	EXPRESSION TAG
Ser -17	-	EXPRESSION TAG
His -16	-	EXPRESSION TAG
Met -15	-	EXPRESSION TAG
Ala -14	-	EXPRESSION TAG
Ser -13	-	EXPRESSION TAG
Met -12	-	EXPRESSION TAG
Thr -11	-	EXPRESSION TAG
Gly -10	-	EXPRESSION TAG
Gly -9	-	EXPRESSION TAG
Gln -8	-	EXPRESSION TAG
Gln -7	-	EXPRESSION TAG
Met -6	-	EXPRESSION TAG
Gly -5	-	EXPRESSION TAG
Arg -4	-	EXPRESSION TAG
Gly -3	-	EXPRESSION TAG
Ser -2	-	EXPRESSION TAG
Glu -1	-	EXPRESSION TAG
Phe 0	-	EXPRESSION TAG
Ser 65	Ala 65	ENGINEERED MUTATION
Gly 89	Ser 89	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	38707.6
Non-Polymers*	Number of molecules	3
	Total formula weight	288.2
All*	Total formula weight	38995.8

*Water molecules are not included.