4H04

Lacto-N-biosidase from Bifidobacterium bifidum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Lacto-N-biosidase	polymer	644	71892.2	2	UniProt (B3TLD6) Pfam (PF02838) Pfam (PF00728) Pfam (PF14200) In PDB	Bifidobacterium bifidum
2	C, D	beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		383.3	2	In PDB GlyTouCan (G00056MO)
3	A, B	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
4		water	water		18.0	1327	Chemie (HOH)

Sequence modifications

A, B: 41 - 663 (UniProt: B3TLD6)

PDB	External Database	Details
Met 20	-	expression tag
Gly 21	-	expression tag
Ser 22	-	expression tag
Ser 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
His 28	-	expression tag
His 29	-	expression tag
Ser 30	-	expression tag
Ser 31	-	expression tag
Gly 32	-	expression tag
Leu 33	-	expression tag
Val 34	-	expression tag
Pro 35	-	expression tag
Arg 36	-	expression tag
Gly 37	-	expression tag
Ser 38	-	expression tag
His 39	-	expression tag
Met 40	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	143784.5
Branched	Number of molecules	2
	Total formula weight	766.7
Non-Polymers*	Number of molecules	8
	Total formula weight	768.5
All*	Total formula weight	145319.7

*Water molecules are not included.