4GZZ
Crystal structures of bacterial RNA Polymerase paused elongation complexes
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 315 | 35056.2 | 2 | UniProt (Q5SHR6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Thermus thermophilus | RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1119 | 125436.5 | 1 | UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Thermus thermophilus | RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1534 | 172378.8 | 1 | UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Thermus thermophilus | RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 99 | 11533.3 | 1 | UniProt (Q8RQE7) Pfam (PF01192) | Thermus thermophilus | RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega |
| 5 | F (N) | non-template DNA | polymer | 13 | 4000.6 | 1 | synthetic | ||
| 6 | G (R) | RNA transcript | polymer | 16 | 5131.1 | 1 | synthetic | ||
| 7 | H (T) | template DNA | polymer | 22 | 6696.3 | 1 | synthetic | ||
| 8 | I, J (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 9 | K (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
D: 1 - 1524 (UniProt: Q8RQE8)
| PDB | External Database | Details |
|---|---|---|
| His 1525 | - | expression tag |
| His 1526 | - | expression tag |
| His 1527 | - | expression tag |
| His 1528 | - | expression tag |
| His 1529 | - | expression tag |
| His 1530 | - | expression tag |
| His 1531 | - | expression tag |
| His 1532 | - | expression tag |
| His 1533 | - | expression tag |
| His 1534 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 395289.0 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 395444.1 |
