4GZI

Active-site mutant of potato endo-1,3-beta-glucanase in complex with laminaratriose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glucan endo-1,3-beta-D-glucosidase	polymer	323	36550.9	1	UniProt (Q70C53) Pfam (PF00332)	Solanum tuberosum (potato)	1,3-beta-D-glucan glucanohydrolase
2	B (B)	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose	branched		504.4	1	In PDB GlyTouCan (G00024MO)
3	C (A)	water	water		18.0	338	Chemie (HOH)

Sequence modifications

A: 24 - 338 (UniProt: Q70C53)

PDB	External Database	Details
Ala 259	Glu 259	engineered mutation
Leu 339	-	expression tag
Asn 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36550.9
Branched	Number of molecules	1
	Total formula weight	504.4
All*	Total formula weight	37055.4

*Water molecules are not included.