4GYP

Crystal structure of the heterotetrameric complex of GlucD and GlucDRP from E. coli K-12 MG1655 (EFI TARGET EFI-506058)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Glucarate dehydratase	polymer	446	49196.9	2	UniProt (P0AES2) Pfam (PF13378)	Escherichia coli	GDH, GlucD
2	C, D (C, D)	Glucarate dehydratase-related protein	polymer	458	50400.0	2	UniProt (Q46915) Pfam (PF02746) Pfam (PF13378)	Escherichia coli	GDH-RP, GlucDRP
3	E, I (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	F, J (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5	G, L, P, Q (A, C, D)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
6	H, M, R (A, C, D)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
7	K, N (C, D)	CITRIC ACID	non-polymer		192.1	2	Chemie (CIT)
8	O (D)	HEXAETHYLENE GLYCOL	non-polymer		282.3	1	Chemie (P6G)
9	S, T, U, V (A, B, C, D)	water	water		18.0	1450	Chemie (HOH)

Sequence modifications

C, D: 2 - 446 (UniProt: Q46915)

PDB	External Database	Details
Met -11	-	expression tag
Gly -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	199193.6
Non-Polymers*	Number of molecules	14
	Total formula weight	1594.1
All*	Total formula weight	200787.7

*Water molecules are not included.