4GX0

Crystal structure of the GsuK L97D mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	TrkA domain protein	polymer	565	61832.2	4	UniProt (Q74FS9) Pfam (PF07885) Pfam (PF02254) Pfam (PF02080) In PDB	Geobacter sulfurreducens
2	E	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	C, D, A, B	POTASSIUM ION	non-polymer		39.1	12	Chemie (K)
4	D, A, B, C	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	C, A, B	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
6	C, D, A, B	PHOSPHATE ION	non-polymer		95.0	5	Chemie (PO4)
7	D, B	alpha-D-glucopyranose	non-polymer		180.2	3	Chemie (GLC)
8		water	water		18.0	391	Chemie (HOH)

Sequence modifications

A, B, C, D: 9 - 564 (UniProt: Q74FS9)

PDB	External Database	Details
Met 4	-	expression tag
Gln 5	-	expression tag
Arg 6	-	expression tag
Gly 7	-	expression tag
Ser 8	-	expression tag
Ala 52	Glu 52	engineered mutation
Glu 77	Gln 77	engineered mutation
Asp 97	Leu 97	engineered mutation
Leu 565	-	expression tag
Val 566	-	expression tag
Pro 567	-	expression tag
Arg 568	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	247329.0
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	28
	Total formula weight	1906.4
All*	Total formula weight	249577.7

*Water molecules are not included.