4GVI

Crystal structure of mutant (D248N) Salmonella typhimurium family 3 glycoside hydrolase (NagZ) in complex with GlcNAc-1,6-anhMurNAc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Beta-hexosaminidase	polymer	349	38722.9	2	UniProt (Q8ZQ06) Pfam (PF00933) In PDB	Salmonella enterica subsp. enterica serovar Typhimurium	Beta-N-acetylhexosaminidase, N-acetyl-beta-glucosaminidase
2	A, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
3	A, B	2-(2-ACETYLAMINO-4-HYDROXY-6,8-DIOXA-BICYCLO[3.2.1]OCT-3-YLOXY)-PROPIONIC ACID	non-polymer		275.3	2	Chemie (AH0)
4	B	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
5		water	water		18.0	783	Chemie (HOH)

Sequence modifications

A, B: 1 - 341 (UniProt: Q8ZQ06)

PDB	External Database	Details
Asn 248	Asp 248	engineered mutation
Gly 342	-	expression tag
Ser 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	77445.9
Non-Polymers*	Number of molecules	5
	Total formula weight	1188.2
All*	Total formula weight	78634.1

*Water molecules are not included.