4GTL

T. Maritima FDTS (R174K mutant) with FAD

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Thymidylate synthase thyX	polymer	232	27475.7	4	UniProt (Q9WYT0) Pfam (PF02511) In PDB	Thermotoga maritima	TS, TSase
2	B, C, D	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	3	Chemie (FAD)
3		water	water		18.0	81	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 220 (UniProt: Q9WYT0)

PDB	External Database	Details
Met -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Asp -8	-	expression tag
Lys -7	-	expression tag
Ile -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Lys 174	Arg 174	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	109902.7
Non-Polymers*	Number of molecules	3
	Total formula weight	2356.6
All*	Total formula weight	112259.3

*Water molecules are not included.