4GTF

T. Maritima FDTS (H53A mutant) with FAD, dUMP and Folate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Thymidylate synthase thyX	polymer	232	27436.6	1	UniProt (Q9WYT0) Pfam (PF02511)	Thermotoga maritima	TS, TSase
2	B (A)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	1	Chemie (FAD)
3	C (A)	2'-DEOXYURIDINE 5'-MONOPHOSPHATE	non-polymer		308.2	1	Chemie (UMP)
4	D (A)	N-({4-[(6aR)-3-amino-1-oxo-1,2,5,6,6a,7-hexahydroimidazo[1,5-f]pteridin-8(9H)-yl]phenyl}carbonyl)-L-glutamic acid	non-polymer		457.4	1	Chemie (MEF)
5	E (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	F (A)	water	water		18.0	124	Chemie (HOH)

Sequence modifications

A: 1 - 220 (UniProt: Q9WYT0)

PDB	External Database	Details
Met -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Asp -8	-	expression tag
Lys -7	-	expression tag
Ile -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 53	His 53	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27436.6
Non-Polymers*	Number of molecules	4
	Total formula weight	1586.6
All*	Total formula weight	29023.2

*Water molecules are not included.