4GRV

The crystal structure of the neurotensin receptor NTS1 in complex with neurotensin (8-13)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Neurotensin receptor type 1, lysozyme chimera	polymer	510	57202.0	1	UniProt (P20789) UniProt (P00720) Pfam (PF00001) Pfam (PF00959)	Rattus norvegicus (Rat Bacteriaphage T4)	NT-R-1, NTR1, High-affinity levocabastine-insensitive neurotensin receptor, NTRH
2	B (B)	Neurotensin 8-13	polymer	6	819.0	1	UniProt (by SIFTS) (P20068)
3	C, D (A)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	2	Chemie (EPE)
4	E (A)	water	water		18.0	23	Chemie (HOH)

Sequence modifications

A: 43 - 268 (UniProt: P20789)

PDB	External Database	Details
Asp 33	-	expression tag
Tyr 34	-	expression tag
Lys 35	-	expression tag
Asp 36	-	expression tag
Asp 37	-	expression tag
Asp 38	-	expression tag
Asp 39	-	expression tag
Ala 40	-	expression tag
Thr 41	-	expression tag
Ser 42	-	expression tag
Leu 86	Ala 86	engineered mutation
Ala 166	Glu 166	engineered mutation
Ala 215	Gly 215	engineered mutation

A: 1002 - 1161 (UniProt: P00720)

PDB	External Database	Details
Thr 1054	Cys 54	engineered mutation
Ala 1097	Cys 97	engineered mutation
Asn 1122	Gln 122	engineered mutation
Asn 1123	Gln 123	engineered mutation
Gly 1162	-	linker
Ser 1163	-	linker
Gly 1164	-	linker
Ser 1165	-	linker

A: 300 - 396 (UniProt: P20789)

PDB	External Database	Details
Ala 310	Leu 310	engineered mutation
Ala 358	Phe 358	engineered mutation
Ala 360	Val 360	engineered mutation
Ala 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag
Gly 408	-	expression tag
Gly 409	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58021.0
Non-Polymers*	Number of molecules	2
	Total formula weight	476.6
All*	Total formula weight	58497.6

*Water molecules are not included.