4GL2

Structural Basis for dsRNA duplex backbone recognition by MDA5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Interferon-induced helicase C domain-containing protein 1	polymer	699	80467.5	2	UniProt (Q9BYX4) Pfam (PF04851) Pfam (PF18119) Pfam (PF00271) Pfam (PF11648) In PDB	Homo sapiens (human)	Clinically amyopathic dermatomyositis autoantigen 140 kDa, CADM-140 autoantigen, Helicase with 2 CARD domains, Helicard, Interferon-induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, MDA-5, Murabutide down-regulated protein, RIG-I-like receptor 2, RLR-2, RNA helicase-DEAD box protein 116
2	C, E	RNA (5'-R(*AP*UP*CP*CP*GP*CP*GP*GP*CP*CP*CP*U)-3')	polymer	12	3763.3	2
3	F, D	RNA (5'-R(P*AP*GP*GP*GP*CP*CP*GP*CP*GP*GP*AP*U)-3')	polymer	12	3906.4	2
4	A, B	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
5	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, B: 306 - 1017 (UniProt: Q9BYX4)

PDB	External Database	Details
Gly 301	-	EXPRESSION TAG
Pro 302	-	EXPRESSION TAG
Gly 303	-	EXPRESSION TAG
Ala 304	-	EXPRESSION TAG
Met 305	-	EXPRESSION TAG
Cys 333	Ser 333	SEE REMARK 999
-	Glu 642	DELETION
-	Asp 643	DELETION
-	Asp 644	DELETION
-	Ser 645	DELETION
-	Asp 646	DELETION
-	Glu 647	DELETION
-	Gly 648	DELETION
-	Gly 649	DELETION
-	Asp 650	DELETION
-	Asp 651	DELETION
-	Glu 652	DELETION
-	Tyr 653	DELETION
-	Cys 654	DELETION
-	Asp 655	DELETION
-	Gly 656	DELETION
-	Asp 657	DELETION
-	Glu 658	DELETION
-	Asp 659	DELETION
Arg 843	His 843	SEE REMARK 999
Thr 946	Ala 946	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	176274.3
Non-Polymers*	Number of molecules	4
	Total formula weight	1143.2
All*	Total formula weight	177417.5

*Water molecules are not included.