4GKK

Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a human mitochondrial anticodon stem loop (ASL) of transfer RNA Methionine (TRNAMET) bound to an mRNA with an AUA-codon in the A-site and paromomycin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	16S rRNA	polymer	1513	491166.8	1	GenBank (48256)	Thermus thermophilus
2	B	30S ribosomal protein S2	polymer	234	26987.3	1	UniProt (P80371) Pfam (PF00318) In PDB	Thermus thermophilus
3	C	30S ribosomal protein S3	polymer	206	22862.4	1	UniProt (P80372) Pfam (PF07650) Pfam (PF00189) In PDB	Thermus thermophilus
4	D	30S ribosomal protein S4	polymer	208	24242.3	1	UniProt (P80373) Pfam (PF00163) Pfam (PF01479) In PDB	Thermus thermophilus
5	E	30S ribosomal protein S5	polymer	150	16331.1	1	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719) In PDB	Thermus thermophilus
6	F	30S ribosomal protein S6	polymer	101	11988.8	1	UniProt (Q5SLP8) Pfam (PF01250) In PDB	Thermus thermophilus
7	G	30S ribosomal protein S7	polymer	155	17919.8	1	UniProt (P17291) Pfam (PF00177) In PDB	Thermus thermophilus
8	H	30S ribosomal protein S8	polymer	138	15868.6	1	UniProt (Q5SHQ2) In PDB	Thermus thermophilus
9	I	30S ribosomal protein S9	polymer	127	14298.5	1	UniProt (P80374) Pfam (PF00380) In PDB	Thermus thermophilus
10	J	30S ribosomal protein S10	polymer	98	11299.2	1	UniProt (Q5SHN7) Pfam (PF00338) In PDB	Thermus thermophilus
11	K	30S ribosomal protein S11	polymer	119	12606.4	1	UniProt (P80376) Pfam (PF00411) In PDB	Thermus thermophilus
12	L	30S ribosomal protein S12	polymer	124	13804.3	1	UniProt (Q5SHN3) Pfam (PF00164) In PDB	Thermus thermophilus
13	M	30S ribosomal protein S13	polymer	125	14207.7	1	UniProt (P80377) Pfam (PF00416) In PDB	Thermus thermophilus
14	N	30S ribosomal protein S14 type Z	polymer	60	7027.5	1	UniProt (Q5SHQ1) In PDB	Thermus thermophilus
15	O	30S ribosomal protein S15	polymer	88	10447.2	1	UniProt (Q5SJ76) Pfam (PF00312) In PDB	Thermus thermophilus
16	P	30S ribosomal protein S16	polymer	83	9924.5	1	UniProt (Q5SJH3) Pfam (PF00886) In PDB	Thermus thermophilus
17	Q	30S ribosomal protein S17	polymer	104	12193.5	1	UniProt (Q5SHP7) In PDB	Thermus thermophilus
18	R	30S ribosomal protein S18	polymer	73	8483.2	1	UniProt (Q5SLQ0) Pfam (PF01084) In PDB	Thermus thermophilus
19	S	30S ribosomal protein S19	polymer	80	9203.7	1	UniProt (Q5SHP2) Pfam (PF00203) In PDB	Thermus thermophilus
20	T	30S ribosomal protein S20	polymer	99	10907.1	1	UniProt (P80380) Pfam (PF01649) In PDB	Thermus thermophilus
21	V	30S ribosomal protein Thx	polymer	24	2960.5	1	UniProt (Q5SIH3) Pfam (PF17070) In PDB	Thermus thermophilus
22	W	mRNA A-site fragment	polymer	6	1907.2	1		Synthetic
23	X	tRNA ASL human mitochondrial Met	polymer	17	5405.3	1		Synthetic
24	A, B, T	MAGNESIUM ION	non-polymer		24.3	186	Chemie (MG)
25	A	PAROMOMYCIN	non-polymer		615.6	1	Chemie (PAR)
26	D, N	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 5 - 1521 (GenBank: 48256)

PDB	External Database	Details
C 1005	-	expression tag
G 1013	-	expression tag
C 1225	-	expression tag
A 1226	-	expression tag
U 1517	C 1508	conflict
U 1519	C 1510	conflict

I: 2 - 128 (UniProt: P80374)

PDB	External Database	Details
Arg 58	His 58	conflict

Q: 2 - 105 (UniProt: Q5SHP7)

PDB	External Database	Details
Gln 96	Glu 96	conflict

R: 16 - 88 (UniProt: Q5SLQ0)

PDB	External Database	Details
Gly 60	Ala 60	conflict

T: 8 - 106 (UniProt: P80380)

PDB	External Database	Details
Val 41	Ile 41	conflict

Contents of the asymmetric unit

Polymers	Number of chains	23
	Total formula weight	772042.6
Non-Polymers*	Number of molecules	189
	Total formula weight	5267.2
All*	Total formula weight	777309.7

*Water molecules are not included.