4GK8

Crystal structure of histidinol phosphate phosphatase (HISK) from Lactococcus lactis subsp. lactis Il1403 complexed with ZN and L-histidinol arsenate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Histidinol-phosphatase	polymer	284	33235.8	1	UniProt (Q02150) Pfam (PF02811) In PDB	Lactococcus lactis subsp. lactis	HolPase
2	A	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
3	A	{[(2S)-2-amino-3-(1H-imidazol-5-yl)propyl]oxy}(trihydroxy)-lambda~5~-arsanyl	non-polymer		266.1	1	Chemie (GK8)
4	A	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
5	A	IMIDAZOLE	non-polymer		69.1	1	Chemie (IMD)
6	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
7		water	water		18.0	181	Chemie (HOH)

Sequence modifications

A: 4 - 271 (UniProt: Q02150)

PDB	External Database	Details
Met 1	-	expression tag
Ser 2	-	expression tag
Leu 3	-	expression tag
Gln 65	Arg 63	engineered mutation
Thr 127	Ile 125	engineered mutation
Arg 217	Lys 215	engineered mutation
Lys 272	-	expression tag
Leu 273	-	expression tag
Ala 274	-	expression tag
Ala 275	-	expression tag
Ala 276	-	expression tag
Leu 277	-	expression tag
Glu 278	-	expression tag
His 279	-	expression tag
His 280	-	expression tag
His 281	-	expression tag
His 282	-	expression tag
His 283	-	expression tag
His 284	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33235.8
Non-Polymers*	Number of molecules	10
	Total formula weight	809.3
All*	Total formula weight	34045.1

*Water molecules are not included.