4GK5

Crystal structure of human Rev3-Rev7-Rev1-Polkappa complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Mitotic spindle assembly checkpoint protein MAD2B	polymer	238	26960.0	2	UniProt (Q9UI95) Pfam (PF02301)	Homo sapiens (human)	Mitotic arrest deficient 2-like protein 2, MAD2-like protein 2, REV7 homolog, hREV7
2	C, D (C, D)	DNA polymerase zeta catalytic subunit	polymer	52	5632.3	2	UniProt (O60673)	Homo sapiens (human)	Protein reversionless 3-like, REV3-like, hREV3
3	E, F (E, F)	DNA repair protein REV1	polymer	136	15179.4	2	UniProt (Q9UBZ9) Pfam (PF16727)	Homo sapiens (human)	Alpha integrin-binding protein 80, AIBP80, Rev1-like terminal deoxycytidyl transferase
4	G (G)	DNA polymerase kappa	polymer	10	1275.5	1	UniProt (Q9UBT6)	Homo sapiens	DINB protein, DINP

Sequence modifications

A, B: 1 - 211 (UniProt: Q9UI95)

PDB	External Database	Details
Met -15	-	expression tag
Gly -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Ser -5	-	expression tag
Gln -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Asn -1	-	expression tag
Ser 0	-	expression tag
Ala 124	Arg 124	engineered mutation
Gly 212	-	expression tag
Ser 213	-	expression tag
Gly 214	-	expression tag
Ser 215	-	expression tag
Gly 216	-	expression tag
Ser 217	-	expression tag
Gly 218	-	expression tag
Ser 219	-	expression tag
Gly 220	-	expression tag
Ser 221	-	expression tag
His 222	-	expression tag

E, F: 1117 - 1251 (UniProt: Q9UBZ9)

PDB	External Database	Details
Met 1116	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	96819.0
All*	Total formula weight	96819.0

*Water molecules are not included.