4GJP

Crystal structure of the TAL effector dHax3 bound to dsDNA containing repetitive methyl-CpG

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, B)	Hax3	polymer	499	51756.5	2	UniProt (Q3ZD72)	Xanthomonas campestris pv. armoraciae
2	B, E (I, G)	DNA (5'-D(*TP*GP*TP*CP*CP*CP*TP*TP*(5CM)P*GP*(5CM)P*GP*TP*CP*TP*CP*T)-3')	polymer	17	5124.3	2
3	C, F (J, H)	DNA (5'-D(*AP*GP*AP*GP*AP*CP*GP*CP*GP*AP*AP*GP*GP*GP*AP*CP*A)-3')	polymer	17	5319.5	2
4	G, H (J, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	I, J, K, L, M... (A, I, J, B, G...)	water	water		18.0	753	Chemie (HOH)

Sequence modifications

A, B: 231 - 720 (UniProt: Q3ZD72)

PDB	External Database	Details
Met 230	-	expression tag
His 300	Asn 300	engineered mutation
Asp 301	Ile 301	engineered mutation
His 368	Asn 368	engineered mutation
Asp 369	Ile 369	engineered mutation
Asn 402	His 402	engineered mutation
Gly 403	Asp 403	engineered mutation
Asn 436	His 436	engineered mutation
Gly 437	Asp 437	engineered mutation
Asn 470	His 470	engineered mutation
Gly 471	Asp 471	engineered mutation
Asn 505	Ser 505	engineered mutation
Gly 539	Ser 539	engineered mutation
Asn 573	Ser 573	engineered mutation
Asn 606	His 606	engineered mutation
Gly 607	Asp 607	engineered mutation
His 640	Asn 640	engineered mutation
Asp 641	Ile 641	engineered mutation
Leu 721	-	expression tag
Glu 722	-	expression tag
His 723	-	expression tag
His 724	-	expression tag
His 725	-	expression tag
His 726	-	expression tag
His 727	-	expression tag
His 728	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	124400.6
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	124449.2

*Water molecules are not included.