4GJJ

Crystal structure of Pseudomonas stutzeri L-rhamnose isomerase mutant H101N in complex with D-allopyranose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	L-rhamnose isomerase	polymer	438	47980.6	4	UniProt (Q75WH8) Pfam (PF01261) In PDB	Pseudomonas stutzeri
2	A, B, C, D	MANGANESE (II) ION	non-polymer		54.9	10	Chemie (MN)
3	A, C, D	D-ALLOSE	non-polymer		180.2	3	Chemie (AOS)
4	B	alpha-D-allopyranose	non-polymer		180.2	1	Chemie (AFD)
5		water	water		18.0	335	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 430 (UniProt: Q75WH8)

PDB	External Database	Details
Asn 101	His 101	engineered mutation
Asn 150	Asp 150	engineered mutation
Gly 431	-	expression tag
Ser 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag
His 438	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	191922.3
Non-Polymers*	Number of molecules	14
	Total formula weight	1270.0
All*	Total formula weight	193192.3

*Water molecules are not included.