4GJI

Crystal structure of Pseudomonas stutzeri L-rhamnose isomerase mutant H101N in complex with L-rhamnopyranose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	L-rhamnose isomerase	polymer	438	47980.6	4	UniProt (Q75WH8) Pfam (PF01261)	Pseudomonas stutzeri
2	E, J, O, U (A, B, C, D)	L-RHAMNOSE	non-polymer		164.2	4	Chemie (RNS)
3	F, K, P, V (A, B, C, D)	alpha-L-rhamnopyranose	non-polymer		164.2	4	Chemie (RAM)
4	G, H, M, N, Q... (A, B, C, D)	MANGANESE (II) ION	non-polymer		54.9	10	Chemie (MN)
5	I, L, T (B, D)	beta-L-rhamnopyranose	non-polymer		164.2	3	Chemie (RM4)
6	AA, BA, CA, Z (B, C, D, A)	water	water		18.0	1964	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 430 (UniProt: Q75WH8)

PDB	External Database	Details
Asn 101	His 101	engineered mutation
Asn 150	Asp 150	engineered mutation
Gly 431	-	expression tag
Ser 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag
His 438	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	191922.3
Non-Polymers*	Number of molecules	21
	Total formula weight	2355.1
All*	Total formula weight	194277.4

*Water molecules are not included.