4GHM
Crystal Structure of the H233A mutant of 7-cyano-7-deazaguanine reductase, QueF from Vibrio cholerae complexed with preQ0
Replaces: 3RZQEntity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | NADPH-dependent 7-cyano-7-deazaguanine reductase | polymer | 290 | 33248.3 | 2 | UniProt (Q9KTK0) Pfam (PF14819) Pfam (PF14489) In PDB | Vibrio cholerae O1 biovar El Tor | QueF, 7-cyano-7-carbaguanine reductase, NADPH-dependent nitrile oxidoreductase, PreQ(0) reductase |
2 | A, B | 7-DEAZA-7-AMINOMETHYL-GUANINE | non-polymer | 179.2 | 2 | Chemie (PRF) | |||
3 | A, B | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
4 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
5 | water | water | 18.0 | 478 | Chemie (HOH) |
Sequence modifications
A, B: 7 - 287 (UniProt: Q9KTK0)
PDB | External Database | Details |
---|---|---|
Ser -2 | - | expression tag |
Asn -1 | - | expression tag |
Ala 0 | - | expression tag |
Mse 1 | - | expression tag |
Asn 2 | - | expression tag |
Arg 3 | - | expression tag |
Leu 4 | - | expression tag |
Lys 5 | - | expression tag |
Asn 6 | - | expression tag |
Ala 233 | His 227 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 66496.5 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 658.9 | |
All* | Total formula weight | 67155.5 |