4GHM

Crystal Structure of the H233A mutant of 7-cyano-7-deazaguanine reductase, QueF from Vibrio cholerae complexed with preQ0

Replaces:  3RZQ

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	NADPH-dependent 7-cyano-7-deazaguanine reductase	polymer	290	33248.3	2	UniProt (Q9KTK0) Pfam (PF14819) Pfam (PF14489)	Vibrio cholerae O1 biovar El Tor	QueF, 7-cyano-7-carbaguanine reductase, NADPH-dependent nitrile oxidoreductase, PreQ(0) reductase
2	C, G (A, B)	7-DEAZA-7-AMINOMETHYL-GUANINE	non-polymer		179.2	2	Chemie (PRF)
3	D, F, H (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
4	E (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	I, J (A, B)	water	water		18.0	478	Chemie (HOH)

Sequence modifications

A, B: 7 - 287 (UniProt: Q9KTK0)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag
Mse 1	-	expression tag
Asn 2	-	expression tag
Arg 3	-	expression tag
Leu 4	-	expression tag
Lys 5	-	expression tag
Asn 6	-	expression tag
Ala 233	His 227	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66496.5
Non-Polymers*	Number of molecules	6
	Total formula weight	658.9
All*	Total formula weight	67155.5

*Water molecules are not included.