4GGH

Crystal structure of an enolase family member from vibrio harveyi (efi-target 501692) with homology to mannonate dehydratase, with mg, hepes, and ethylene glycol bound (ordered loops, space group c2221)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Putative uncharacterized protein	polymer	422	48225.2	4	UniProt (D0X4R4) Pfam (PF02746) Pfam (PF13378) In PDB	Vibrio harveyi
2	D, A, B, C	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
3	D, A, B, C	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	4	Chemie (EPE)
4	D, A, B, C	1,2-ETHANEDIOL	non-polymer		62.1	15	Chemie (EDO)
5	C, A	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
6	D, C	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
7		water	water		18.0	1694	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 399 (UniProt: D0X4R4)

PDB	External Database	Details
Met -22	-	initiating methionine
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
Gly -13	-	expression tag
Val -12	-	expression tag
Asp -11	-	expression tag
Leu -10	-	expression tag
Gly -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	192900.8
Non-Polymers*	Number of molecules	30
	Total formula weight	2331.8
All*	Total formula weight	195232.5

*Water molecules are not included.