4GEQ

Crystal structure of the Spc24-Spc25/Cnn1 binding interface

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Kinetochore protein SPC25	polymer	90	9889.3	2	UniProt (P40014)	Saccharomyces cerevisiae S288c (yeast)
2	B, D (B, D)	Kinetochore protein SPC24	polymer	66	7903.0	2	UniProt (Q04477) Pfam (PF08286)	Saccharomyces cerevisiae S288c (yeast)
3	E, F (E, F)	Kinetochore-associated protein CNN1	polymer	25	2848.1	2	UniProt (P43618)	Saccharomyces cerevisiae S288c	Co-purified with NNF1 protein 1
4	G, H (B, C)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	I, J, K, L, M... (A, B, C, D, E...)	water	water		18.0	150	Chemie (HOH)

Sequence modifications

A, C: 133 - 221 (UniProt: P40014)

PDB	External Database	Details
Met 132	-	expression tag

B, D: 155 - 213 (UniProt: Q04477)

PDB	External Database	Details
Met 154	-	expression tag
His 214	-	expression tag
His 215	-	expression tag
His 216	-	expression tag
His 217	-	expression tag
His 218	-	expression tag
His 219	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	41280.9
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	41465.1

*Water molecules are not included.