4GEO

P38a MAP kinase DEF-pocket penta mutant (M194A, L195A, H228A, I229A, Y258A)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Mitogen-activated protein kinase 14	polymer	367	41939.7	1	UniProt (Q16539) Pfam (PF00069) In PDB	Homo sapiens (human)	p38a, MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, CSBP, MAP kinase MXI2, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, MAP kinase p38 alpha, Stress-activated protein kinase 2a, SAPK2a
2	A	octyl beta-D-glucopyranoside	non-polymer		292.4	2	Chemie (BOG)
3		water	water		18.0	286	Chemie (HOH)

Sequence modifications

A: 2 - 360 (UniProt: Q16539)

PDB	External Database	Details
Met -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 1	-	expression tag
Ala 194	Met 194	engineered mutation
Ala 195	Leu 195	engineered mutation
Ala 228	His 228	engineered mutation
Ala 229	Ile 229	engineered mutation
Ala 258	Tyr 258	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	41939.7
Non-Polymers*	Number of molecules	2
	Total formula weight	584.7
All*	Total formula weight	42524.4

*Water molecules are not included.