4GEG

Crystal Structure of E.coli MenH Y85F Mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase	polymer	268	29499.3	3	UniProt (P37355) Pfam (PF12697) In PDB	Escherichia coli	SHCHC synthase
2	A, B, C	SULFATE ION	non-polymer		96.1	13	Chemie (SO4)
3	A, B, C	CHLORIDE ION	non-polymer		35.5	7	Chemie (CL)
4	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5	B, C	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6		water	water		18.0	419	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 252 (UniProt: P37355)

PDB	External Database	Details
Met -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Phe 85	Tyr 85	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	88498.0
Non-Polymers*	Number of molecules	23
	Total formula weight	1743.2
All*	Total formula weight	90241.3

*Water molecules are not included.