4GEC

Crystal Structure of E.coli MenH R124A Mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase	polymer	268	29429.2	3	UniProt (P37355) Pfam (PF12697)	Escherichia coli	SHCHC synthase
2	D, E, I, K (A, B, C)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	F (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	G, J, L (A, B, C)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
5	H (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
6	M, N, O (A, B, C)	water	water		18.0	232	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 252 (UniProt: P37355)

PDB	External Database	Details
Met -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 124	Arg 124	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	88287.7
Non-Polymers*	Number of molecules	9
	Total formula weight	644.8
All*	Total formula weight	88932.5

*Water molecules are not included.