4GD3
Structure of E. coli hydrogenase-1 in complex with cytochrome b
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G (S, T, Q, R) | Hydrogenase-1 small chain | polymer | 335 | 36820.7 | 4 | UniProt (P69739) Pfam (PF01058) Pfam (PF14720) | Escherichia coli | HYD1, Membrane-bound hydrogenase 1 small subunit, NiFe hydrogenase |
| 2 | B, D, F, H (L, M, J, K) | Hydrogenase-1 large chain | polymer | 582 | 64751.4 | 4 | UniProt (P0ACD8) Pfam (PF00374) | Escherichia coli | HYD1, Membrane-bound hydrogenase 1 large subunit, NiFe hydrogenase |
| 3 | I, J (A, B) | Ni/Fe-hydrogenase 1 B-type cytochrome subunit | polymer | 235 | 27626.3 | 2 | UniProt (P0AAM1) Pfam (PF01292) | Escherichia coli | |
| 4 | CA, DA, K, L, LA... (Q, S, R, T) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 8 | Chemie (SF4) | |||
| 5 | EA, M, NA, V (Q, S, R, T) | FE4-S3 CLUSTER | non-polymer | 319.6 | 4 | Chemie (F4S) | |||
| 6 | BA, FA, GA, KA, N... (M, Q, J, S, K...) | CHLORIDE ION | non-polymer | 35.5 | 10 | Chemie (CL) | |||
| 7 | HA, P, QA, Y (J, L, K, M) | CARBONMONOXIDE-(DICYANO) IRON | non-polymer | 135.9 | 4 | Chemie (FCO) | |||
| 8 | IA, Q, RA, Z (J, L, K, M) | NICKEL (II) ION | non-polymer | 58.7 | 4 | Chemie (NI) | |||
| 9 | AA, JA, R, SA (M, J, L, K) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 10 | OA, VA, W, XA (R, A, T, B) | DODECYL-BETA-D-MALTOSIDE | non-polymer | 510.6 | 4 | Chemie (LMT) | |||
| 11 | UA, WA (A, B) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 2 | Chemie (HEM) | |||
| 12 | AB, BB, CB, DB, EB... (T, M, Q, J, R...) | water | water | 18.0 | 40 | Chemie (HOH) |
Sequence modifications
S, T, Q, R: 1 - 327 (UniProt: P69739)
| PDB | External Database | Details |
|---|---|---|
| Cys 242 | Pro 287 | engineered mutation |
| Arg 328 | - | expression tag |
| Ser 329 | - | expression tag |
| His 330 | - | expression tag |
| His 331 | - | expression tag |
| His 332 | - | expression tag |
| His 333 | - | expression tag |
| His 334 | - | expression tag |
| His 335 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 461540.9 | |
| Non-Polymers* | Number of molecules | 40 |
| Total formula weight | 8596.9 | |
| All* | Total formula weight | 470137.8 |
