4G7Z

Crystal structure of Thermus thermophilus transcription initiation complex containing 5-BrU at template-strand position +1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, K, L	DNA-directed RNA polymerase subunit alpha	polymer	315	35056.2	4	UniProt (Q5SHR6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Thermus thermophilus	RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha
2	C, M	DNA-directed RNA polymerase subunit beta	polymer	1119	125436.5	2	UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB	Thermus thermophilus	RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta
3	D, N	DNA-directed RNA polymerase subunit beta'	polymer	1524	170997.4	2	UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Thermus thermophilus	RNA polymerase beta-prime subunit, RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta'
4	E, O	DNA-directed RNA polymerase subunit omega	polymer	99	11533.3	2	UniProt (Q8RQE7) Pfam (PF01192) In PDB	Thermus thermophilus	RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega
5	F, P	RNA polymerase sigma factor	polymer	443	50769.4	2	UniProt (Q5SKW1) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Thermus thermophilus	RNA polymerase principle sigma factor, sigma A
6	H, R	5'-D(*TP*AP*TP*AP*AP*TP*GP*GP*GP*AP*GP*CP*TP*GP*TP*CP*AP*CP*GP*GP*AP*TP*GP*CP*AP*GP*G)-3'	polymer	27	8421.4	2
7	G, Q	5'-D(*CP*CP*TP*GP*CP*AP*TP*CP*CP*GP*TP*GP*AP*GP*(BRU)P*CP*GP*AP*GP*GP*G)-3'	polymer	21	6545.0	2
8	D, N	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
9	D, N	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

F, P: 1 - 423 (UniProt: Q5SKW1)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	887630.9
Non-Polymers*	Number of molecules	6
	Total formula weight	310.2
All*	Total formula weight	887941.1

*Water molecules are not included.