4G4J
Crystal structure of glucuronoyl esterase S213A mutant from Sporotrichum thermophile in complex with methyl 4-O-methyl-beta-D-glucopyranuronate determined at 2.35 A resolution
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | 4-O-methyl-glucuronoyl methylesterase | polymer | 433 | 46046.2 | 1 | UniProt (G2QJR6) In PDB | Myceliophthora thermophila | |
2 | A | methyl 4-O-methyl-beta-D-glucopyranuronate | non-polymer | 222.2 | 1 | Chemie (MCU) | |||
3 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 20 | Chemie (EDO) | |||
4 | A | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
5 | water | water | 18.0 | 212 | Chemie (HOH) |
Sequence modifications
A: 1 - 397 (UniProt: G2QJR6)
PDB | External Database | Details |
---|---|---|
Ser -13 | - | insertion |
Met -12 | - | insertion |
Ala -11 | - | insertion |
Pro -10 | - | insertion |
Met -9 | - | insertion |
Asn -8 | - | insertion |
His -7 | - | insertion |
Ile -6 | - | insertion |
Phe -5 | - | insertion |
Glu -4 | - | insertion |
Arg -3 | - | insertion |
Gln -2 | - | insertion |
Asp -1 | - | insertion |
Thr 0 | - | insertion |
Ala 213 | Ser 213 | engineered mutation |
Leu 398 | - | SEE REMARK 999 |
Glu 399 | - | SEE REMARK 999 |
Gln 400 | - | SEE REMARK 999 |
Lys 401 | - | SEE REMARK 999 |
Leu 402 | - | SEE REMARK 999 |
Ile 403 | - | SEE REMARK 999 |
Ser 404 | - | SEE REMARK 999 |
Glu 405 | - | SEE REMARK 999 |
Glu 406 | - | SEE REMARK 999 |
Asp 407 | - | SEE REMARK 999 |
Leu 408 | - | SEE REMARK 999 |
Asn 409 | - | SEE REMARK 999 |
Ser 410 | - | SEE REMARK 999 |
Ala 411 | - | SEE REMARK 999 |
Val 412 | - | SEE REMARK 999 |
Asp 413 | - | SEE REMARK 999 |
His 414 | - | expression tag |
His 415 | - | expression tag |
His 416 | - | expression tag |
His 417 | - | expression tag |
His 418 | - | expression tag |
His 419 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 46046.2 | |
Non-Polymers* | Number of molecules | 23 |
Total formula weight | 1647.7 | |
All* | Total formula weight | 47693.9 |