4G37
Structure of cross-linked firefly luciferase in second catalytic conformation
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Luciferin 4-monooxygenase | polymer | 555 | 61016.1 | 2 | UniProt (P08659) Pfam (PF00501) Pfam (PF13193) | Photinus pyralis (North American firefly) | Luciferase |
| 2 | C, G (A, B) | 5'-O-[N-(DEHYDROLUCIFERYL)-SULFAMOYL] ADENOSINE | non-polymer | 606.6 | 2 | Chemie (SLU) | |||
| 3 | D, H (A, B) | 4,4'-(ethylenediimino)bis[4-oxobutyrate] | non-polymer | 274.3 | 2 | Chemie (XLX) | |||
| 4 | E, F, J, K (A, B) | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 5 | I (B) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | L, M (A, B) | water | water | 18.0 | 190 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 550 (UniProt: P08659)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Leu -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ser 81 | Cys 81 | engineered mutation |
| Cys 108 | Ile 108 | engineered mutation |
| Ala 214 | Thr 214 | engineered mutation |
| Leu 215 | Ala 215 | engineered mutation |
| Ala 216 | Cys 216 | engineered mutation |
| Ala 232 | Ile 232 | engineered mutation |
| Ser 258 | Cys 258 | engineered mutation |
| Leu 295 | Phe 295 | engineered mutation |
| Lys 354 | Glu 354 | engineered mutation |
| Ser 391 | Cys 391 | engineered mutation |
| Cys 447 | Tyr 447 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 122032.3 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 2181.5 | |
| All* | Total formula weight | 124213.7 |
