4FZW

Crystal Structure of the PaaF-PaaG Hydratase-Isomerase Complex from E.coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	2,3-dehydroadipyl-CoA hydratase	polymer	258	27638.8	2	UniProt (P76082) Pfam (PF00378)	Escherichia coli	Enoyl-CoA hydratase
2	C, D (C, D)	1,2-epoxyphenylacetyl-CoA isomerase	polymer	274	29772.0	2	UniProt (P77467) Pfam (PF00378)	Escherichia coli
3	E, F, G (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
4	H, I, J, K (A, B, C, D)	water	water		18.0	121	Chemie (HOH)

Sequence modifications

A, B: 1 - 255 (UniProt: P76082)

PDB	External Database	Details
Met -2	-	initiating methionine
Arg -1	-	expression tag
Ser 0	-	expression tag

C, D: 1 - 262 (UniProt: P77467)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	114821.6
Non-Polymers*	Number of molecules	3
	Total formula weight	276.3
All*	Total formula weight	115097.9

*Water molecules are not included.