4FXF

Structure of M2 pyruvate kinase in complex with phenylalanine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, D, C, B)	Pyruvate kinase isozymes M1/M2	polymer	551	60164.2	4	UniProt (P14618) Pfam (PF00224) Pfam (PF02887)	Homo sapiens (human)	Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58
2	E, I, O, S (A, D, C, B)	OXALATE ION	non-polymer		88.0	4	Chemie (OXL)
3	F, J, P, T (A, D, C, B)	POTASSIUM ION	non-polymer		39.1	4	Chemie (K)
4	G, K, M, Q, U... (A, D, C, B)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
5	H, N, R, X (A, D, C, B)	1,6-di-O-phosphono-beta-D-fructofuranose	non-polymer		340.1	4	Chemie (FBP)
6	L, V (D, B)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
7	AA, BA, Y, Z (C, B, A, D)	water	water		18.0	428	Chemie (HOH)

Sequence modifications

A, D, C, B: 1 - 531 (UniProt: P14618)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asn 379	His 379	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	240656.8
Non-Polymers*	Number of molecules	20
	Total formula weight	3029.1
All*	Total formula weight	243685.9

*Water molecules are not included.