4FTW
Crystal structure of a carboxyl esterase N110C/L145H at 2.3 angstrom resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Phospholipase/Carboxylesterase | polymer | 285 | 29919.9 | 1 | UniProt (Q3J2V1) Pfam (PF02230) | Rhodobacter sphaeroides | |
| 2 | B (A) | PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID) | non-polymer | 302.4 | 1 | Chemie (PIN) | |||
| 3 | C (A) | 3-CYCLOHEXYLPROPYL 4-O-ALPHA-D-GLUCOPYRANOSYL-BETA-D-GLUCOPYRANOSIDE | non-polymer | 466.5 | 1 | Chemie (3CM) | |||
| 4 | D (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 5 | E (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | F (A) | water | water | 18.0 | 22 | Chemie (HOH) |
Sequence modifications
A: 49 - 268 (UniProt: Q3J2V1)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | expression tag |
| His 2 | - | expression tag |
| His 3 | - | expression tag |
| His 4 | - | expression tag |
| His 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| Ser 8 | - | expression tag |
| Ser 9 | - | expression tag |
| Gly 10 | - | expression tag |
| Leu 11 | - | expression tag |
| Val 12 | - | expression tag |
| Pro 13 | - | expression tag |
| Arg 14 | - | expression tag |
| Gly 15 | - | expression tag |
| Ser 16 | - | expression tag |
| Gly 17 | - | expression tag |
| Met 18 | - | expression tag |
| Lys 19 | - | expression tag |
| Glu 20 | - | expression tag |
| Thr 21 | - | expression tag |
| Ala 22 | - | expression tag |
| Ala 23 | - | expression tag |
| Ala 24 | - | expression tag |
| Lys 25 | - | expression tag |
| Phe 26 | - | expression tag |
| Glu 27 | - | expression tag |
| Arg 28 | - | expression tag |
| Gln 29 | - | expression tag |
| His 30 | - | expression tag |
| Met 31 | - | expression tag |
| Asp 32 | - | expression tag |
| Ser 33 | - | expression tag |
| Pro 34 | - | expression tag |
| Asp 35 | - | expression tag |
| Leu 36 | - | expression tag |
| Gly 37 | - | expression tag |
| Thr 38 | - | expression tag |
| Asp 39 | - | expression tag |
| Asp 40 | - | expression tag |
| Asp 41 | - | expression tag |
| Asp 42 | - | expression tag |
| Lys 43 | - | expression tag |
| Ala 44 | - | expression tag |
| Met 45 | - | expression tag |
| Ala 46 | - | expression tag |
| Asp 47 | - | expression tag |
| Ile 48 | - | expression tag |
| Cys 110 | Asn 62 | engineered mutation |
| His 145 | Leu 97 | engineered mutation |
| Ala 269 | - | expression tag |
| Cys 270 | - | expression tag |
| Gly 271 | - | expression tag |
| Arg 272 | - | expression tag |
| Thr 273 | - | expression tag |
| Arg 274 | - | expression tag |
| Ala 275 | - | expression tag |
| Pro 276 | - | expression tag |
| Pro 277 | - | expression tag |
| Pro 278 | - | expression tag |
| Pro 279 | - | expression tag |
| Pro 280 | - | expression tag |
| Leu 281 | - | expression tag |
| Arg 282 | - | expression tag |
| Ser 283 | - | expression tag |
| Gly 284 | - | expression tag |
| Cys 285 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 29919.9 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 827.3 | |
| All* | Total formula weight | 30747.2 |
