4FQ5

Crystal structure of the maleate isomerase Iso(C200A) from Pseudomonas putida S16 with maleate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	Maleate cis-trans isomerase	polymer	281	30572.2	2	UniProt (F8G0M3) Pfam (PF17645)	Pseudomonas putida
2	C, D (B, A)	MALEIC ACID	non-polymer		116.1	2	Chemie (MAE)
3	E, F (B, A)	water	water		18.0	299	Chemie (HOH)

Sequence modifications

B, A: 2 - 252 (UniProt: F8G0M3)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Val 1	-	expression tag
Ala 200	Cys 200	engineered mutation
Leu 253	-	expression tag
Glu 254	-	expression tag
His 255	-	expression tag
His 256	-	expression tag
His 257	-	expression tag
His 258	-	expression tag
His 259	-	expression tag
His 260	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61144.4
Non-Polymers*	Number of molecules	2
	Total formula weight	232.1
All*	Total formula weight	61376.5

*Water molecules are not included.