4FNS
Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D | Alpha-galactosidase AgaA | polymer | 729 | 83299.9 | 4 | UniProt (Q9ALJ4) Pfam (PF16875) Pfam (PF02065) Pfam (PF16874) In PDB | Geobacillus stearothermophilus | |
| 2 | A, B, C, D | (2R,3S,4R,5S)-2-(hydroxymethyl)piperidine-3,4,5-triol | non-polymer | 163.2 | 4 | Chemie (DGJ) | |||
| 3 | A, B, C, D | SULFATE ION | non-polymer | 96.1 | 8 | Chemie (SO4) | |||
| 4 | D | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 5 | water | water | 18.0 | 980 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 729 (UniProt: Q9ALJ4)
| PDB | External Database | Details |
|---|---|---|
| Glu 355 | Ala 355 | engineered mutation |
| Leu 518 | Phe 518 | engineered mutation |
| Val 704 | Met 704 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 333199.4 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1483.3 | |
| All* | Total formula weight | 334682.7 |
