4FM2

Pyrococcus abyssi B family DNA polymerase (triple mutant) bound to a dsDNA, in edition mode

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	T	Template strand	polymer	15	4667.0	1		synthetic construct
2	P	Primer strand	polymer	8	2411.6	1		synthetic construct
3	A	DNA polymerase 1	polymer	793	91663.9	1	UniProt (P0CL77) Pfam (PF03104) Pfam (PF00136) In PDB	Pyrococcus abyssi	Pab polymerase
4	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6		water	water		18.0	139	Chemie (HOH)

Sequence modifications

A: 1 - 771 (UniProt: P0CL77)

PDB	External Database	Details
Met -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Leu -8	-	expression tag
Val -7	-	expression tag
Pro -6	-	expression tag
Ala -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
His -2	-	expression tag
Ala -1	-	expression tag
Gly 0	-	expression tag
Ala 4	Asp 4	engineered mutation
Ala 215	Asp 215	engineered mutation
Ala 251	Glu 251	engineered mutation
Ala 343	Asp 343	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	98742.5
Non-Polymers*	Number of molecules	2
	Total formula weight	116.4
All*	Total formula weight	98858.9

*Water molecules are not included.