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4FLZ

Pyrococcus abyssi B family DNA polymerase bound to a dsDNA, in edition mode

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)
DNA polymerase 1polymer79391809.91UniProt (P0CL77)
Pfam (PF03104)
Pfam (PF00136)
Pyrococcus abyssiPab polymerase
2B
(P)
Primer strandpolymer82411.61synthetic construct
3C
(T)
Template strandpolymer154659.01synthetic construct
4D, E
(A)
MAGNESIUM IONnon-polymer24.32Chemie (MG)
5F
(A)
GLYCEROLnon-polymer92.11Chemie (GOL)
6G, H
(A, T)
waterwater18.066Chemie (HOH)
Sequence modifications
A: 1 - 771 (UniProt: P0CL77)
PDBExternal DatabaseDetails
Met -21-expression tag
Gly -20-expression tag
Ser -19-expression tag
Ser -18-expression tag
His -17-expression tag
His -16-expression tag
His -15-expression tag
His -14-expression tag
His -13-expression tag
His -12-expression tag
Ser -11-expression tag
Ser -10-expression tag
Gly -9-expression tag
Leu -8-expression tag
Val -7-expression tag
Pro -6-expression tag
Ala -5-expression tag
Gly -4-expression tag
Ser -3-expression tag
His -2-expression tag
Ala -1-expression tag
Gly 0-expression tag
Ala 215Asp 215engineered mutation
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains3
Total formula weight98880.6
Non-Polymers*Number of molecules3
Total formula weight140.7
All*Total formula weight99021.3
*Water molecules are not included.

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PDB entries from 2024-11-06

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