4FLT
Pyrococcus abyssi B family DNA polymerase bound to a dsDNA, in edition mode
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | DNA polymerase 1 | polymer | 793 | 91809.9 | 1 | UniProt (P0CL77) Pfam (PF03104) Pfam (PF00136) In PDB | Pyrococcus abyssi | Pab polymerase |
2 | P | Primer strand | polymer | 11 | 3399.2 | 1 | synthetic construct | ||
3 | T | Template strand | polymer | 13 | 4031.6 | 1 | synthetic construct | ||
4 | A | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
5 | A | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
6 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
7 | water | water | 18.0 | 313 | Chemie (HOH) |
Sequence modifications
A: 1 - 771 (UniProt: P0CL77)
PDB | External Database | Details |
---|---|---|
Met -21 | - | expression tag |
Gly -20 | - | expression tag |
Ser -19 | - | expression tag |
Ser -18 | - | expression tag |
His -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
Ser -11 | - | expression tag |
Ser -10 | - | expression tag |
Gly -9 | - | expression tag |
Leu -8 | - | expression tag |
Val -7 | - | expression tag |
Pro -6 | - | expression tag |
Ala -5 | - | expression tag |
Gly -4 | - | expression tag |
Ser -3 | - | expression tag |
His -2 | - | expression tag |
Ala -1 | - | expression tag |
Gly 0 | - | expression tag |
Ala 215 | Asp 215 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 99240.8 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 335.9 | |
All* | Total formula weight | 99576.7 |