4FFI
Crystal Structure of Levan Fructotransferase D54N mutant from Arthrobacter ureafaciens in complex with levanbiose
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Levan fructotransferase | polymer | 492 | 54387.9 | 4 | UniProt (Q9KJD0) Pfam (PF00251) Pfam (PF08244) In PDB | Arthrobacter ureafaciens | |
2 | E, F, G, I, J... | beta-D-fructofuranose-(2-6)-beta-D-fructofuranose | branched | 342.3 | 9 | In PDB BIRD (PRD_900051) GlyTouCan (G51829YA) | levanbiose | ||
3 | H | beta-D-fructofuranose-(2-6)-beta-D-fructofuranose-(2-6)-beta-D-fructofuranose | branched | 504.4 | 1 | In PDB BIRD (PRD_900063) GlyTouCan (G30220AI) | levantriose | ||
4 | water | water | 18.0 | 847 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 41 - 521 (UniProt: Q9KJD0)
PDB | External Database | Details |
---|---|---|
Met 40 | - | expression tag |
Asn 54 | Asp 54 | engineered mutation |
Asp 115 | Gly 115 | conflict |
Leu 522 | - | expression tag |
Glu 523 | - | expression tag |
His 524 | - | expression tag |
His 525 | - | expression tag |
His 526 | - | expression tag |
His 527 | - | expression tag |
His 528 | - | expression tag |
His 529 | - | expression tag |
His 530 | - | expression tag |
His 531 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 217551.4 | |
Branched | Number of molecules | 10 |
Total formula weight | 3585.1 | |
All* | Total formula weight | 221136.5 |