4FFH

Crystal Structure of Levan Fructotransferase D54N mutant from Arthrobacter ureafaciens in complex with sucrose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Levan fructotransferase	polymer	492	54387.9	4	UniProt (Q9KJD0) Pfam (PF00251) Pfam (PF08244) In PDB	Arthrobacter ureafaciens
2	E, F, G, H, I...	beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose	branched		342.3	6	In PDB BIRD (PRD_900003)		sucrose
3		water	water		18.0	837	Chemie (HOH)

Sequence modifications

A, B, C, D: 41 - 521 (UniProt: Q9KJD0)

PDB	External Database	Details
Met 40	-	expression tag
Asn 54	Asp 54	engineered mutation
Asp 115	Gly 115	conflict
Leu 522	-	expression tag
Glu 523	-	expression tag
His 524	-	expression tag
His 525	-	expression tag
His 526	-	expression tag
His 527	-	expression tag
His 528	-	expression tag
His 529	-	expression tag
His 530	-	expression tag
His 531	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	217551.4
Branched	Number of molecules	6
	Total formula weight	2053.8
All*	Total formula weight	219605.2

*Water molecules are not included.