4F7I

Structure of Isopropylmalate dehydrogenase from Thermus thermophilus in complex with IPM, Mn and NADH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	3-isopropylmalate dehydrogenase	polymer	359	38398.9	4	UniProt (Q5SIY4) Pfam (PF00180)	Thermus thermophilus	3-IPM-DH, Beta-IPM dehydrogenase, IMDH
2	E, HA, U, WA (A, C, B, D)	3-ISOPROPYLMALIC ACID	non-polymer		176.2	4	Chemie (IPM)
3	F, IA, V, XA (A, C, B, D)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
4	G, JA, YA (A, C, D)	3[N-MORPHOLINO]PROPANE SULFONIC ACID	non-polymer		209.3	3	Chemie (MPO)
5	H, KA, W, ZA (A, C, B, D)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
6	AB, I, J, LA, MA... (D, A, C, B)	POTASSIUM ION	non-polymer		39.1	7	Chemie (K)
7	AA, BA, BB, CA, CB... (B, D, A, C)	GLYCEROL	non-polymer		92.1	29	Chemie (GOL)
8	FA, HB, IB, T, UA... (B, D, A, C)	ETHANOL	non-polymer		46.1	6	Chemie (EOH)
9	JB, KB, LB, MB (A, B, C, D)	water	water		18.0	440	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 345 (UniProt: Q5SIY4)

PDB	External Database	Details
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ala 346	-	expression tag
Ala 347	-	expression tag
Ala 348	-	expression tag
Leu 349	-	expression tag
Glu 350	-	expression tag
His 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag
His 354	-	expression tag
His 355	-	expression tag
His 356	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	153595.5
Non-Polymers*	Number of molecules	57
	Total formula weight	7426.7
All*	Total formula weight	161022.3

*Water molecules are not included.