4F72
Crystal structure of had family enzyme bt-2542 (target efi-501088) from Bacteroides thetaiotaomicron, asp12ala mutant, complex with magnesium and inorganic phosphate
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Putative haloacid dehalogenase-like hydrolase | polymer | 229 | 26777.8 | 2 | UniProt (Q8A4Q5) Pfam (PF00702) In PDB | Bacteroides thetaiotaomicron | |
2 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
3 | A, B | PHOSPHATE ION | non-polymer | 95.0 | 3 | Chemie (PO4) | |||
4 | A, B | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
5 | water | water | 18.0 | 40 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 207 (UniProt: Q8A4Q5)
PDB | External Database | Details |
---|---|---|
Met -21 | - | expression tag |
His -20 | - | expression tag |
His -19 | - | expression tag |
His -18 | - | expression tag |
His -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
Ser -14 | - | expression tag |
Ser -13 | - | expression tag |
Gly -12 | - | expression tag |
Val -11 | - | expression tag |
Asp -10 | - | expression tag |
Leu -9 | - | expression tag |
Gly -8 | - | expression tag |
Thr -7 | - | expression tag |
Glu -6 | - | expression tag |
Asn -5 | - | expression tag |
Leu -4 | - | expression tag |
Tyr -3 | - | expression tag |
Phe -2 | - | expression tag |
Gln -1 | - | expression tag |
Ser 0 | - | expression tag |
Ala 12 | Asp 12 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 53555.6 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 517.7 | |
All* | Total formula weight | 54073.3 |