4F4J

Conversion of the enzyme guanylate kinase into a mitotic spindle orienting protein by a single mutation that inhibits gmp- induced closing

Replaces:  3SQK

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Guanylate kinase	polymer	202	22419.2	2	UniProt (P15454) Pfam (PF00625)	Saccharomyces cerevisiae (Baker's yeast)	GMP kinase
2	C, D, E, F, G (A, B)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
3	H, I (A, B)	water	water		18.0	91	Chemie (HOH)

Sequence modifications

A, B: 1 - 187 (UniProt: P15454)

PDB	External Database	Details
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Pro 35	Ser 35	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	44838.4
Non-Polymers*	Number of molecules	5
	Total formula weight	480.3
All*	Total formula weight	45318.7

*Water molecules are not included.