4F2U

Structure of the N254Y/H258Y double mutant of the Phosphatidylinositol-Specific Phospholipase C from S.aureus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	1-phosphatidylinositol phosphodiesterase	polymer	310	35316.2	1	UniProt (P45723) Pfam (PF00388) In PDB	Staphylococcus aureus subsp. aureus	Phosphatidylinositol diacylglycerol-lyase, Phosphatidylinositol-specific phospholipase C, PI-PLC
2	A	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	2	Chemie (EPE)
3	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4		water	water		18.0	288	Chemie (HOH)

Sequence modifications

A: 1 - 302 (UniProt: P45723)

PDB	External Database	Details
Tyr 254	Asn 264	engineered mutation
Tyr 258	His 268	engineered mutation
Leu 303	-	expression tag
Glu 304	-	expression tag
His 305	-	expression tag
His 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	35316.2
Non-Polymers*	Number of molecules	3
	Total formula weight	572.7
All*	Total formula weight	35888.9

*Water molecules are not included.