4EMQ

Crystal structure of a single mutant of Dronpa, the green-on-state PDM1-4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Fluorescent protein Dronpa	polymer	257	29231.9	6	UniProt (Q5TLG6) Pfam (PF01353) In PDB	Echinophyllia sp. SC22
2	A, B, C, D, E...	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	6	Chemie (EPE)
3	A	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
4	B, C, D, E, F	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	7	Chemie (PEG)
5		water	water		18.0	884	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 224 (UniProt: Q5TLG6)

PDB	External Database	Details
Met -32	-	expression tag
Arg -31	-	expression tag
Gly -30	-	expression tag
Ser -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
Gly -22	-	expression tag
Met -21	-	expression tag
Ala -20	-	expression tag
Ser -19	-	expression tag
Met -18	-	expression tag
Thr -17	-	expression tag
Gly -16	-	expression tag
Gly -15	-	expression tag
Gln -14	-	expression tag
Gln -13	-	expression tag
Met -12	-	expression tag
Gly -11	-	expression tag
Arg -10	-	expression tag
Asp -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Lys -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Gyc 63	Cys 62	chromophore
Gyc 63	Tyr 63	chromophore
Gyc 63	Gly 64	chromophore
Asn 145	Lys 145	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	175391.3
Non-Polymers*	Number of molecules	14
	Total formula weight	2410.9
All*	Total formula weight	177802.3

*Water molecules are not included.