4EFX

Highly biologically active insulin with additional disulfide bond

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Insulin	polymer	21	2373.7	2	UniProt (P01308)	Homo sapiens (human)	Insulin A chain
2	B, D (B, D)	Insulin	polymer	28	3178.7	2	UniProt (P01308)	Homo sapiens (human)	Insulin B chain
3	E, F (B, D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	G, H, I, J (A, B, C, D)	water	water		18.0	33	Chemie (HOH)

Sequence modifications

A, C: 1 - 21 (UniProt: P01308)

PDB	External Database	Details
Cys 10	Ile 99	engineered mutation

B, D: 1 - 28 (UniProt: P01308)

PDB	External Database	Details
Cys 4	Gln 28	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	11104.7
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	11235.5

*Water molecules are not included.