4EEA
Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta1,4-Glc-BETA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Beta-1,4-galactosyltransferase 1 | polymer | 287 | 32773.2 | 3 | UniProt (P15291) Pfam (PF13733) Pfam (PF02709) | Homo sapiens (human) | Beta-1,4-GalTase 1, Beta4Gal-T1, b4Gal-T1, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1, Lactose synthase A protein, N-acetyllactosamine synthase, Nal synthase, Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase, Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase, Processed beta-1,4-galactosyltransferase 1 |
| 2 | D, E, F (D, E, F) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-6)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose | branched | 545.5 | 3 | In PDB GlyTouCan (G83540EL) | |||
| 3 | BA, G, P (C, A, B) | URIDINE-5'-DIPHOSPHATE | non-polymer | 404.2 | 3 | Chemie (UDP) PubChem (1158) PubChem (6031) PubChem (3084486) PubChem (6101632) PubChem (6604175) PubChem (11811538) PubChem (12049241) PubChem (25323044) PubChem (25323048) PubChem (44298965) PubChem (57448939) PubChem (53486216) PubChem (60098857) PubChem (91746160) PubChem (92332560) PubChem (92332561) PubChem (92332562) PubChem (92332563) PubChem (93088131) PubChem (93630083) | |||
| 4 | CA, H, Q (C, A, B) | MANGANESE (II) ION | non-polymer | 54.9 | 3 | Chemie (MN) PubChem (27854) | |||
| 5 | DA, I, R, S (C, A, B) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) PubChem (753) | |||
| 6 | AA, EA, FA, GA, HA... (B, C, A) | SULFATE ION | non-polymer | 96.1 | 20 | Chemie (SO4) | |||
| 7 | KA, LA, MA (A, B, C) | water | water | 18.0 | 724 | Chemie (HOH) |
Sequence modifications
A, B, C: 126 - 398 (UniProt: P15291)
| PDB | External Database | Details |
|---|---|---|
| Ala 112 | - | expression tag |
| Ser 113 | - | expression tag |
| Met 114 | - | expression tag |
| Thr 115 | - | expression tag |
| Gly 116 | - | expression tag |
| Gly 117 | - | expression tag |
| Gln 118 | - | expression tag |
| Gln 119 | - | expression tag |
| Met 120 | - | expression tag |
| Gly 121 | - | expression tag |
| Arg 122 | - | expression tag |
| Gly 123 | - | expression tag |
| Ser 124 | - | expression tag |
| Ala 125 | - | expression tag |
| Thr 337 | Arg 337 | engineered mutation |
| Thr 338 | Cys 338 | engineered mutation |
| His 340 | Met 340 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 98319.7 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1636.5 | |
| Non-Polymers* | Number of molecules | 30 |
| Total formula weight | 3666.9 | |
| All* | Total formula weight | 103623.1 |
