4ECE

Crystal structure of purine nucleoside phosphorylase (W16Y, W94Y, W178Y, H257W) mutant from human complexed with guanine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Purine nucleoside phosphorylase	polymer	324	36127.1	6	UniProt (P00491) Pfam (PF01048)	Homo sapiens (human)	PNP, Inosine phosphorylase
2	G, H, I, J, K... (A, B, C, D, E...)	GUANINE	non-polymer		151.1	6	Chemie (GUN)
3	M, N, O, P, Q... (A, B, C, D, E...)	water	water		18.0	305	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 289 (UniProt: P00491)

PDB	External Database	Details
Met -34	-	initiating methionine
Arg -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
Gly -24	-	expression tag
Met -23	-	expression tag
Ala -22	-	expression tag
Ser -21	-	expression tag
Met -20	-	expression tag
Thr -19	-	expression tag
Gly -18	-	expression tag
Gly -17	-	expression tag
Gln -16	-	expression tag
Gln -15	-	expression tag
Met -14	-	expression tag
Gly -13	-	expression tag
Arg -12	-	expression tag
Asp -11	-	expression tag
Leu -10	-	expression tag
Tyr -9	-	expression tag
Asp -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Thr -1	-	expression tag
Leu 0	-	expression tag
Tyr 16	Trp 16	engineered mutation
Ser 51	Gly 51	engineered mutation
Tyr 94	Trp 94	engineered mutation
Tyr 178	Trp 178	engineered mutation
Trp 257	His 257	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	216762.8
Non-Polymers*	Number of molecules	6
	Total formula weight	906.8
All*	Total formula weight	217669.5

*Water molecules are not included.